Abstract: 3D-Printed Bioactive Ceramic Scaffolds Demonstrate Intrinsic Osteogenic Properties in an Undisturbed Osseous Environment
نویسندگان
چکیده
METHODS: Fat was harvested by manual liposuction and processed by standard Coleman technique from 24 patients undergoing fat grafting to the forefoot as part of a randomized cross-over clinical trial. Ultrasound-assessed submetatarsal tissue thickness was obtained at baseline, 6mo, & 12mo visits. Processed lipoaspirate was returned to the lab for stromal vascular fraction (SVF) isolation, flow cytometry characterization, collagen assessment using western blot, and histological imaging.
منابع مشابه
Systematical Evaluation of Mechanically Strong 3D Printed Diluted magnesium Doping Wollastonite Scaffolds on Osteogenic Capacity in Rabbit Calvarial Defects
Wollastonite (CaSiO3; CSi) ceramic is a promising bioactive material for bone defect repair due to slightly fast degradation of its porous constructs in vivo. In our previous strategy some key features of CSi ceramic have been significantly improved by dilute magnesium doping for regulating mechanical properties and biodegradation. Here we demonstrate that 6 ~ 14% of Ca substituted by Mg in CSi...
متن کاملNano-biphasic calcium phosphate/polyvinyl alcohol composites with enhanced bioactivity for bone repair via low-temperature three-dimensional printing and loading with platelet-rich fibrin
Background and aim As a newly emerging three-dimensional (3D) printing technology, low-temperature robocasting can be used to fabricate geometrically complex ceramic scaffolds at low temperatures. Here, we aimed to fabricate 3D printed ceramic scaffolds composed of nano-biphasic calcium phosphate (BCP), polyvinyl alcohol (PVA), and platelet-rich fibrin (PRF) at a low temperature without the add...
متن کامل[Article] Key role of the expression of bone morphogenetic proteins in increasing the osteogenic activity of osteoblast-like cells exposed to shock waves and seeded on bioactive glass-ceramic scaffolds for bone tissue engineering
In this work, the role of shock-wave (SW)-induced increase of bone morphogenetic proteins (BMPs) in modulating the osteogenic properties of osteoblast-like cells seeded on a bioactive scaffold was investigated using gremlin as a BMP antagonist. Bone-like glass-ceramic scaffolds, based on a silicate experimental bioactive glass (CEL2) developed at the Politecnico di Torino, were produced by the ...
متن کاملBioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface
The cartilage lesion resulting from osteoarthritis (OA) always extends into subchondral bone. It is of great importance for simultaneous regeneration of two tissues of cartilage and subchondral bone. 3D-printed Sr5(PO4)2SiO4 (SPS) bioactive ceramic scaffolds may achieve the aim of regenerating both of cartilage and subchondral bone. We hypothesized that strontium (Sr) and silicon (Si) ions rele...
متن کاملIncreased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration
Background: One of the main challenges with conventional scaffold fabrication methods is the inability to control scaffold architecture. Recently, scaffolds with controlled shape and architecture have been fabricated using 3D-printing. Herein, we aimed to determine whether the much tighter control of microstructure of 3DP PLGA/β-TCP scaffolds is more effective in promoting osteogenesis than por...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 5 شماره
صفحات -
تاریخ انتشار 2017